Anti-Melanogenic Effects of Paederia foetida L. Extract via MAPK Signaling-Mediated MITF Downregulation

In this study, in order to explore the anti-melanogenic effect of PFE (Paederia foetida L. extract) and suggest its availability, B16F10 cells, which are murine melanoma were stimulated with alpha-Melanocyte-stimulating hormone (α-MSH) conduct an vitro experiment. Treatment cells activated melanogenesis due stimulants showed that significantly inhibits melanin content as well intracellular tyrosinase activity within a range does not cause cytotoxicity. addition, Western blot assay demonstrated strongly inhibited protein expression only tyrosinase-related (TRP)-1, -2, tyrosinase, but also microphthalmia-associated transcription factor (MITF). Moreover, mechanism studies have shown processing activation production by regulating phosphorylation each mitogen-activated kinase (MAPK) family MAPK signaling pathway. To test biocompatibility on human skin, primary skin irritation was performed. The results revealed did any side effects skin. These findings holds great potential whitening agent prevention hyperpigmentation disorders.